Journal: Frontiers in Bioengineering and Biotechnology
Article Title: Effects of buffer composition and plasmid toxicity on electroporation-based non-viral gene delivery in mammalian cells using bursts of nanosecond and microsecond pulses
doi: 10.3389/fbioe.2024.1430637
Figure Lengend Snippet: CHO-K1 cells viability dependence on EP buffers composition (No. 1, 2, 5, and 7) and applied protocols with pEGFP-N1 plasmid encoding green fluorescent protein (GFP). Dot line indicates transfection efficacy using 1.2 kV/cm × 100 μs × 8 protocol. CTRL refers to cells with plasmid in different buffer without electroporation treatment. Asterisk (*) corresponds to statistically significant differences ( p < 0.05).
Article Snippet: Electroctroporation experiments were performed using a 3 kV, 100 ns–1 m square-wave pulse generator (VilniusTECH, Vilnius, Lithuania) ( ) and a commercially available electroporation cuvette with a 1 mm gap between electrodes (Biorad, Hercules, United States).
Techniques: Plasmid Preparation, Transfection, Electroporation